Coding

Part:BBa_K199125:Experience

Designed by: Olivia Ho-Shing   Group: iGEM09_MoWestern_Davidson   (2010-03-16)

Applications of BBa_K199125

This part was designed to be part of a positive feedback loop. We had the gene synthesized by GeneArt and they optimized to codons for use in E. coli. We compared the wt LuxI from V. fischeri and our optimized LuxI. We had one cell type function as a sender cell, and another cell function as a receiver cell. We produced two different sender cells and tested them separately for the ability to induce GFP in the receiver cells. We tested each cell type individually +/- IPTG to induce the pLac promoters.

K199125.png

This part was used in a positive feedback loop part number K199135 and it works as designed.

User Reviews

UNIQ6fe6356ab53f096d-partinfo-00000000-QINU UNIQ6fe6356ab53f096d-partinfo-00000001-QINU